09/01/2024
🐶 𝐈𝐦𝐩𝐫𝐨𝐯𝐞𝐦𝐞𝐧𝐭 𝐨𝐟 𝐒𝐩𝐞𝐫𝐦 𝐌𝐨𝐭𝐢𝐥𝐢𝐭𝐲 𝐰𝐢𝐭𝐡𝐢𝐧 𝐎𝐧𝐞 𝐌𝐨𝐧𝐭𝐡 𝐮𝐧𝐝𝐞𝐫 𝐒𝐞𝐥𝐞𝐧𝐢𝐮𝐦 𝐚𝐧𝐝 𝐕𝐢𝐭𝐚𝐦𝐢𝐧 𝐄 𝐬𝐮𝐩𝐩𝐥𝐞𝐦𝐞𝐧𝐭𝐚𝐭𝐢𝐨𝐧 𝐢𝐧 𝐅𝐨𝐮𝐫 𝐈𝐧𝐟𝐞𝐫𝐭𝐢𝐥𝐞 𝐃𝐨𝐠𝐬 𝐰𝐢𝐭𝐡 𝐋𝐨𝐰 𝐒𝐞𝐥𝐞𝐧𝐢𝐮𝐦 𝐒𝐭𝐚𝐭𝐮𝐬 🐕
🌱 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598180/
The following males were used in the study: a Golden Retriever (I; 36 kg b.w.), an English Cocker Spaniel (II; 23 kg b.w.), and two Tibetan Mastiffs (III; 62 kg b.w.; and IV; 64 kg. b.w.). They were referred to the Clinic in the Department of Animal Reproduction because of conception failure in their last three matings with different bi***es. Before the failed matings these males had at least one litter. The ages of the dogs ranged from three to six years. The patients were in good general condition with normal sexual libido (typical sexual behaviour during natural mating and no problems with semen collection if artificial insemination (AI) was performed), without any disorders or abnormalities of the ge***al tract, no internal diseases or diseases which raised body temperature were stated in the patients’ histories, and no drugs or hormonal treatment had been used. The dogs were fed various commercial dry feeds. The prostate gland and te**es were examined by ultrasonography (8.0 MHz probe MyLab30Gold; Esoate, USA) and did not show any pathological condition.
The dogs were supplemented daily with Se (6 μg/kg of organic selenium from yeast) and vit. E (5 mg/kg) per os for 60 days (𝐒𝐞𝐦𝐞𝐯𝐞𝐭 - 𝐕𝐄𝐓𝐄𝐗𝐏𝐄𝐑𝐓). The preparation also contained 50 mg of evening primrose extract.
Semen was collected by manual manipulation as described by Linde-Forsberg (24) in the presence of a teaser bitch in heat on days 0, 30, 60, and 90. The ej******es were collected into a prewarmed (36–38°C) glass tube.
The s***m concentration and motility indicators were assessed by an IVOS S***m Analyser, version 12.3 (Hamilton, USA). Ej******es were diluted to 50 × 106 s***matozoa/mL with TRIS extender directly before analysis. The following parameters were measured: concentration (CONC), the percentage of motile s***matozoa (MOT), the percentage of s***matozoa with a progressive motility (PMOT), velocity average pathway (VAP), velocity straight line (VSL), velocity curvilinear (VCL), amplitude lateral head (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN), and rapid, medium, slow, and static motility subcategories.
The percentages of live and dead s***matozoa were estimated on dried smears stained with eosin/nigrosin. For the assessment of s***m morphology, monochromatic Diff-Quick stain was used. Two hundred s***matozoa were evaluated per slide, representing 100% of the sample.
These four cases described showed that supplementation with Se and vit. E in dogs with low Se status leads to the rapid improvement of semen motility after only one month followed by an increase in s***m concentration and restoration of fertility. Studies on a larger number of dogs and on individuals with different histories of infertility are still in process and will be the subject of further publications.
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