Giardiasis; cause, symptom, treatment and prevention in dogs

31/10/2022

Giardia is a microscopic parasite that lives in your dog's intestines. From time to time, they form cysts, which your dog passes in their stool. These cysts live in the environment and will infect any animal or human who ingests them unknowingly.

There are many species of Giardia, but the most important is Giardia intestinalis (also known as Giardia duodenalis and Giardia lamblia). This is the species that infects mammals.

31/10/2022

How is giardiasis treated?
The most common drugs used to kill Giardia are fenbendazole and metronidazole. These drugs are normally given for three to ten days to treat giardiasis. Both drugs may be given in combination if necessary. This combination is usually administered to dogs with refractory diarrhea (diarrhea that hasn't responded to treatment). Supportive treatment with other drugs may be needed as supplemental therapy if dehydration or severe diarrhea is present. A low-residue, highly digestible diet may help lessen loose stools during treatment. Some dogs may require follow-up tests and treatments based on their condition and severity of infection. All infected pets should be re-tested two to four weeks after completion of treatment. Your veterinarian will help determine what course of treatment is best for your pet.

02/10/2022

Dogs infected with Giardia experience a spectrum of clinical signs, and diagnostic testing is recommended primarily for symptomatic dogs. At the present time, the molecular evidence suggests that dogs are a relatively minor source of human infection. Giardia is a ubiquitous pathogen and should continue to be a diagnostic differential in cases of both acute and chronic diarrhea.

02/10/2022

Treatment of Giardia in Dogs
No drugs are approved for the treatment of giardiasis in dogs in the United States. The Companion Animal Parasite Council recommends the following:6
Metronidazole (10 to 25 mg/kg q12h for 5 to 8 days) or
Fenbendazole (50 mg/kg q24h for 3 to 5 days) or
A combination of fenbendazole (50 mg/kg q24h) and metronidazole (25 mg/kg q12h) for 5 days
Fenbendazole is approved for the treatment of giardiasis in dogs in Europe. The use of a combination product consisting of febantel, praziquantel, and pyrantel temporarily stopped cyst shedding in dogs,7 but there are not much additional data supporting this treatment.
Albendazole has been used in the treatment of giardiasis; however, there is apprehension about using this drug owing to potential bone marrow toxicity. Albendazole toxicity has been reported in humans, cats, pigs, camelids, and a variety of birds, although it is still used in some of these species.8 In one study, administration of 4 doses of 25 mg/kg was successful in eliminating cyst shedding in dogs with no apparent toxic effects.9 The evidence for toxicity stems from 17 adverse event reports to the Food and Drug Administration Center for Veterinary Medicine (FDA-CVM),10 a published report of suspected toxicosis in a single dog and a single cat,11 and 4 reports of adverse experiences to the United States Pharmacopeia (USP) veterinary practitioner’s reporting program.10 Neither FDA-CVM nor USP recorded complaints associated with use of albendazole in cats. Given its apparent usefulness in treating giardiasis, the use of albenzadole deserves to be re-examined.
Follow-up for Giardia
The primary goal of giardiasis treatment is to resolve diarrhea. Elimination of infection is desired but is much more difficult to achieve. As such, it may be advisable to forgo additional diagnostic testing after a course of treatment and the resolution of clinical signs.
Follow-up investigation can be done 24 to 48 hours after the completion of treatment.6 It is wise to use centrifugal f***l flotation for detection of cysts instead of an ELISA or antigen detection kits since asymptomatic animals may be antigen-positive for some time. Infections that are refractory to treatment may be a sign of immune compromise or another underlying disease condition.
Prevention and Control of Giardia
Preventing and controlling giardiasis can be a difficult task; the cysts persist in the environment and on the hair coat of dogs. Some steps can help decrease, but not eliminate, the risk of reinfection or transmission to other dogs. For example, dogs can be bathed to remove cysts in the hair coat. F***s should be disposed of daily in a manner that does not expose other animals. Surfaces can be cleaned with boiling water or steam.

02/10/2022

Canine Giardiasis
Pathogenesis and Clinical Signs of Giardia
The pathogenesis of giardiasis in dogs is not completely elucidated, and there are likely multiple pathways by which parasites can cause disease. Alterations of normal flora, production of toxins, blunting of microvilli, inhibition of enzymes, and hypersecretion of chloride ions have all been proposed.5 More recently, there has been interest in apoptosis of enterocytes following trophozoite attachment. In this model, attachment leads to alterations in caspase and apoptotic pathways, resulting in degradation of intercellular junctions and barrier dysfunction.5 Affected dogs experience a spectrum of clinical signs ranging from asymptomatic to severely affected, with disease manifesting in acute, intermittent, or chronic timeframes. The insult to enterocytes results in maldigestive malabsorptive diarrhea with an increase in intestinal motility. On the other hand, a significant proportion of dogs are without clinical signs.

Diagnosis of Giardia in Dogs
Testing symptomatic (intermittently or consistently diarrheic) dogs is recommended, using a combination of direct smears, centrifugal f***l flotation, and antigen detection or f***l enzyme-linked immunosorbent assays (ELISAs).6 Repeated testing over multiple days may be required to reveal infection. Examination of f***s will reveal cysts (FIGURE 1) and, much less commonly, trophozoites (FIGURE 2). Zinc sulfate flotation is preferred by some diagnosticians because it does not distort cysts. The osmotic pressure of saturated sugar solution can distort cysts and give them the appearance of a deflated ping-pong ball

02/10/2022

Giardia duodenalis is a flagellated protozoan parasite with a cosmopolitan distribution. It is a common parasite of dogs as well as other mammals (e.g., cats, ungulates, rodents). The taxonomy of Giardia species has been in flux, and the most recent molecular investigations describe both zoonotic and host-adapted isolates of G duodenalis. This article briefly describes the parasite, management of giardiasis, and an update on the zoonotic potential of G duodenalis isolated from dogs.
Stages of Giardia
Giardia trophozoites (tropho, feeding; zoite, life/animal) are the form of the parasite that attaches to enteric epithelial cells. While trophozoites are not frequently found in stool, they are occasionally present in diarrhea. Trophozoites differentiate into a cyst stage before being passed into the environment in f***s. The cyst stage is considered the infectious form and must be ingested by a subsequent host. Cysts are common and may be found in soil, water, or possibly the hair coat of dogs.
Prevalence of Giardia
Giardia is ubiquitous, and dozens of studies have aimed to describe its prevalence in dogs. In some populations the prevalence can be 45% or greater, with specific outbreaks reported in kennels affecting most dogs present.1 Importantly, many dogs lack clinical signs. Giardia infects dogs throughout the world and needs to be considered in the differential diagnosis for diarrhea.
Giardia Host Association
Host specificity of Giardia species has long been a matter of interest, and the understanding of Giardia population structure has improved substantially over the past 20 years. Genetic studies have suggested that G duodenalis is a single species that consists of at least 8 genotypes (A through H), typically referred to as assemblages.2 Assemblages A and B are found in humans and animals, and some, but not all, subtypes within these assemblages are considered zoonotic. Other assemblages appear to have a higher level of host adaptation, with C and D predominating in dogs. Assemblages C and D are not yet reported in humans.
Giardia assemblages can be further divided into subassemblages (I, II, II, etc.); some of the subassemblages are identified as zoonotic. Unfortunately, many of the past studies precede the practice of subtyping, and fewer studies have this level of resolution. Subtyping studies have revealed subassemblages AI, AII, and BIII/IV in humans and dogs;3 however, these reports are sparse (14 dogs total).4
The role of dogs in the transmission of human giardiasis is unresolved, but the data currently available suggest that most human infections are anthroponotic. Besides the few cases of dogs and humans in close proximity with similar subassemblages, there is not enough evidence to make comprehensive conclusions about shedding of zoonotic Giardia by dogs. The bulk of the current molecular evidence suggests that dogs are mainly infected by dog-adapted isolates that are less likely to be transmitted to humans. Still, the concern for a potential zoonosis should be communicated to clients, particularly those susceptible to infection, such as the immunosuppressed.
Transmission of Giardia Between Hosts
At the time of this writing, there is little molecular evidence to suggest that dogs are major sources of human infection. Dogs are most likely to be infected by ingesting cysts shed by other dogs.

02/10/2022

Can my dog give a Giardia infection to me or my family?
Giardia can cause diarrhea in humans and can potentially be passed from dogs to humans. In the past, it was assumed that cats and dogs, along with wildlife, were an important source of infection for humans. Genotype A can infect humans, dogs, and cats while B can infect both humans and dogs.
"...contaminated municipal water supplies are responsible for many outbreaks.”
However, human-to-human transmission is also important and contaminated municipal water supplies are responsible for many outbreaks.
If your dog is diagnosed with giardiasis, environmental disinfection and good personal hygiene are important to prevent accidental spread to humans. In particular, people with immunodeficiency, such as AIDS or cancer, or who are undergoing chemotherapy, should use extreme care, especially when handling f***s or after administering medications.
For environmental disinfection, you can use chlorine bleach at 1:32 or 1:16 dilutions, or 1-2 cups in a gallon of water (60-120 ml/L). However, be sure that the affected surfaces can be safely treated with bleach. Lysol® and quaternary ammonium compounds (Parvosol®, etc.) are also reported to be effective in killing the cysts. Giardia cysts are susceptible to drying so try to keep your environment as dry as possible. For best results, thoroughly clean the pet's living and sleeping areas and then allow the areas to dry out for several days before reintroducing pets.

02/10/2022

What is the prognosis for giardiasis?
The prognosis is good in most cases. Debilitated or geriatric animals and those with incompetent immune systems are at increased risk for complications, including death. All pets diagnosed with Giardia should be re-tested two to four weeks after completion of treatment.

02/10/2022

How is giardiasis treated?
The most common drugs used to kill Giardia are fenbendazole and metronidazole. These drugs are normally given for three to ten days to treat giardiasis. Both drugs may be given in combination if necessary. This combination is usually administered to dogs with refractory diarrhea (diarrhea that hasn't responded to treatment). Supportive treatment with other drugs may be needed as supplemental therapy if dehydration or severe diarrhea is present. A low-residue, highly digestible diet may help lessen loose stools during treatment. Some dogs may require follow-up tests and treatments based on their condition and severity of infection. All infected pets should be re-tested two to four weeks after completion of treatment. Your veterinarian will help determine what course of treatment is best for your pet.
Because Giardia cysts are infective immediately when passed into the environment, f***s should be removed quickly and disposed of. Infected animals should be bathed regularly to remove cysts from the hair coat.

02/10/2022

How is giardiasis diagnosed?
"If your veterinarian suspects giardiasis,
a sample of stool may be a**lyzed
for the presence of
Giardia
specific antigens."
A routine f***l flotation test may fail to detect these tiny cysts, which are shed inconsistently in the f***s, and which often require a special zinc sulfate flotation solution for detection. Occasionally, the parasites may be seen on a direct smear of the f***s. If your veterinarian suspects giardiasis, a sample of stool may be a**lyzed for the presence of Giardia specific antigens (cell proteins). Some Giardia tests are available for in-clinic use while other tests require submittal to a reference laboratory. Many cases are presumptively diagnosed on the basis of medical history and clinical signs suggestive of giardiasis.

02/10/2022

The disease is not usually life threatening
unless the dog’s immune system is immature
or immunocompromised."
The diarrhea may be intermittent. Most dogs do not have a fever but may be less active. The disease is not usually life- threatening unless the dog’s immune system is immature or immunocompromised. Many dogs will be asymptomatic carriers, never developing any signs of illness. Younger animals are more likely to exhibit clinical signs.

02/10/2022

How do dogs get giardiasis?
A dog becomes infected with Giardia when it swallows the cyst stage of the parasite. In susceptible dogs, once the cyst passes into the dog's intestines, it goes through a transformation to the trophozoite, or feeding form, and attaches to the intestinal wall to feed. If sufficient numbers are present, clinical signs of damage to the intestinal wall will develop.
Trophozoites reproduce by dividing, and some transform into the cystic form. Eventually, the dog passes infectious cysts in its stool. The time it takes from ingestion of cysts to passage in f***s is 5 to 12 days in dogs and 5 to 16 days in cats.
"Giardiasis can be transmitted by eating or
sniffing the cysts from contaminated ground,
or by drinking contaminated water."
These cysts are immediately able to infect another animal. Giardiasis can be transmitted by eating or sniffing the cysts from contaminated ground, or by drinking contaminated water.
giardia_in_dogs
When Giardia cysts are found in the stool of a healthy adult dog without diarrhea, they are generally considered a transient, insignificant finding. However, in puppies and debilitated adult dogs, they may cause severe, watery diarrhea that may be fatal if left untreated.
The likelihood of developing disease increases when large numbers of cysts are present in the environment from f***l contamination. Giardiasis is a common occurrence in environments that are densely populated, such as kennels, pet stores, or animal shelters.
What are the clinical signs of giardiasis?
These microscopic parasites attach themselves to the intestinal wall and the damage causes an acute, sudden-onset of foul- smelling diarrhea. Giardia infection in dogs may lead to weight loss, chronic intermittent diarrhea, and fatty stool. The stool may range from soft to watery, often has a greenish tinge to it, and occasionally contains blood. Infected dogs tend to have excess mucus in the f***s. Vomiting may occur in some cases. The signs may persist for several weeks and gradual weight loss may become apparent.

02/10/2022

Giardia is a simple one-celled parasitic species; it is not a "worm", bacteria or virus. There are seven genotypes, A through G, with dogs being most commonly infected by C and D, cats with F, and humans most commonly infected with A and B. Genotype E and F are rarely reported. The parasite occurs worldwide and is a common cause of "Traveler's Diarrhea" in people. Outdoor enthusiasts who inadvertently consume contaminated water may develop "beaver fever", which is another name for giardiasis in people. Other examples of protozoan parasites that can cause enteric (intestinal) disease are Coccidia, Cryptosporidia and Toxoplasma.
Giardiasis can be an important cause of illness, especially diarrhea, in animals and people. However, the majority of dogs infected with Giardia do not have diarrhea, vomiting, or any other signs of illness.
The Giardia organism has two forms. A fragile feeding form, known as the trophozoite, exists in the gut of infected animals, while a hardy cystic form is shed in f***s and can survive several months in the environment, particularly in water and damp environments.

02/06/2022

28/07/2021

Epidemiology
Giardia duodenalis (also known as Giardia lamblia or Giardia intestinalis) has a worldwide distribution, and giardiasis due to G. duodenalis is the most common nationally reportable intestinal parasitic disease identified by public health laboratories in the United States.1 Giardiasis surveillance data show a bimodal age distribution, with the greatest number of reported cases occurring in children aged 1 to 9 years and adults aged 35 to 44 years. In the United States, most cases are reported between early summer and early fall and are associated with recreational water activities (e.g., swimming) and camping.1

Humans are the principal reservoir of G. duodenalis. The parasite is found in many animals species, although the role of zoonotic transmission is still being unraveled.2-4 G. duodenalis is a flagellated protozoan with two forms: trophozoites and cysts. The infectious and environmentally resistant form is the cyst. After ingestion, each Giardia cyst produces two trophozoites in the proximal portion of the small intestine. Detached trophozoites pass through the intestinal tract, and form smooth, oval-shaped, thin-walled infectious cysts that are passed in f***s. Duration of cyst excretion is usually self-limited but can vary and excretion may last for months. Studies in adults have shown that ingestion of as few as 10 to 100 f***lly derived cysts is sufficient to initiate infection.5 Giardia cysts are infectious immediately upon being excreted in f***s and remain viable for at least 3 months in water at 4°C.6 Although freezing will not eliminate the infectivity of Giardia cysts completely, heating, drying, or submersing them in seawater likely will.6,7

Infection with Giardia can occur directly by the f***l-oral route or indirectly via ingestion of contaminated water or food, but water contaminated with Giardia cysts appears to be the major reservoir and vehicle for spread of the parasite.1,8 Most waterborne giardiasis outbreaks have been related to ingestion of untreated or improperly treated surface water.9,10 Drinking untreated mountain stream water is a risk for hikers. Person-to-person spread of giardiasis occurs frequently in child care centers and in families of children with diarrhea.11-13 Antigiardial host defenses are B-cell dependent, with secretory immunoglobulin A playing a major role in immunity. Individuals with humoral immunodeficiencies, such as X-linked agammaglobulinemia and hypogammaglobulinemia, who develop giardiasis are predisposed to chronic symptomatic disease.14

G. duodenalis infection is more common in certain high-risk groups, including children, employees and attendees of child care centers, patients and staff of institutions for people with developmental disabilities, men who have s*x with men, people who ingest contaminated drinking water or recreational water (e.g., water from lakes, rivers, or inadequately treated swimming pools), travelers to disease-endemic areas of the world, close contacts of people with Giardia, people taking antibiotics,13 and people exposed to Giardia-infected domestic and wild animals (e.g., dogs, cats, cattle, deer, and beavers).12,13,15 There is little information on giardiasis in children with HIV infection, although Giardia has been associated with diarrhea in children with HIV infection and AIDS.16,17 A recent study in Kenya described the association of enteric pathogens with HIV infection and HIV exposure in children. Giardia was the second most frequently associated pathogen, but the prevalence of Giardia was similar between the children with HIV and those exposed to HIV.18

Symptoms of giardiasis in individuals with HIV infection appear to be no more severe than in individuals who are HIV negative, and giardiasis is not typically considered a major cause of enteritis in patients with HIV.19 There are no data in individuals with HIV but without advanced disease to suggest that the duration of parasite shedding or length of illness differs from that in individuals who are HIV negative. However, with progressive immunosuppression and reduced CD4 T-lymphocyte (CD4) cell counts, the risk of symptomatic Giardia infections increases. Studies in adults have demonstrated that enteritis due to G. duodenalis is a frequent event among patients with AIDS, especially in the most advanced stage of disease.20 Research in adults with HIV infection from countries where giardiasis is endemic demonstrated that risk of Giardia infections and severity of disease increased with increasing immunosuppression and lower CD4 counts.21,22 In a study of 75 adults with HIV infection in India, G. duodenalis was the most commonly isolated parasite, and patients with lower CD4 counts presented with significantly more enteric disease and chronic diarrhea.23 In another study of 43 adults naive to combination antiretroviral therapy (ART), G. duodenalis was detected in one-third of patients and was significantly associated with lower CD4 counts (OR = 3.0 for CD4 counts ≤100 cells/mm3).24 A cohort study comparing giardiasis in adults with HIV infection in Brazil before and after the introduction of ART demonstrates that the incidence of enteric diseases caused by Giardia decreased after ART was introduced.21 Given the evidence, it is reasonable to recommend initiation of ART and immune reconstitution as a primary mode of Giardia prevention, which is consistent with standard practice to treat all children with HIV infection in the United States with ART.

Clinical Manifestations

The Giardia incubation period usually lasts 1 to 2 weeks and averages 7 days.12 Symptomatic infection with G. duodenalis can cause a broad spectrum of clinical manifestations. Children usually present with short-lasting, acute watery diarrhea with or without low-grade fever, nausea, anorexia, and abdominal pain. In others, the infection has a more protracted intermittent course, characterized by foul-smelling stools associated with flatulence, abdominal distension, and anorexia. Malabsorption combined with anorexia can lead to significant weight loss, failure to thrive, and malnutrition in children.25-27 Stools can initially be profuse and watery and later become greasy and foul smelling. Blood, mucus, and f***l leukocytes are absent. Varying degrees of malabsorption can occur, and abnormal stool patterns can alternate with periods of constipation and normal bowel movements. Post-Giardia infection lactose intolerance can occur in 20% to 40% of patients.28 This syndrome may take several weeks to resolve and can contribute to malnutrition in children. Malnutrition and repeated episodes of Giardia infection in the first years of life have been associated with poor cognitive function in late childhood.29,30 Additionally, a proportion of patients in whom G. duodenalis is diagnosed will also develop chronic GI symptoms such as post infections irritable bowel syndrome (PI-IBS).30

Asymptomatic Giardia infection is common.31 Extraintestinal invasion can occur with trophozoites migrating into bile or pancreatic ducts. Extraintestinal manifestations were previously considered unusual, but recent evidence demonstrates that one third of patients will express long term extraintestinal symptoms, including ocular, muscular and metabolic complications.30 Subsequent development of reactive arthritis has also been associated with giardiasis.32,33

Diagnosis

Although diagnostic tests for Giardia infection have not been evaluated in children with HIV, the tests are expected to perform similarly as in other populations. A definitive diagnosis of Giardia infection is established by detection of Giardia trophozoites or cysts in stool specimens, duodenal fluid, or small-bowel tissue by microscopic examination using staining methods such as trichrome; direct fluorescent antibody (DFA) assays; detection of soluble stool antigens using enzyme immunoassays (EIA); or use of molecular techniques including polymerase chain reaction (PCR).34-36 EIA or multiplex PCR testing is the currently recommended methodology based on assay performance.36

Identification of both trophozoites and cysts can be made on direct smears of concentrated specimens of stool. Appropriately conducted direct examination of stool establishes the diagnosis of Giardia in up to 70% of patients with a single examination and in 85% with a second examination. Identification of Giardia can be difficult because of intermittent excretion of cysts. Stool specimens should be examined within 1 hour after being passed. Trophozoites are more likely to be present in unformed stools because of rapid bowel transit time. Cysts, but not trophozoites, are stable outside the gastrointestinal (GI) tract.

When giardiasis is suspected, and stool specimens are negative, aspiration, biopsy, or both, of the duodenum or upper part of the jejunum should be performed. In a fresh specimen, trophozoites usually can be visualized on direct wet mount. Histologic evaluation of duodenal biopsy samples has low sensitivity for detecting infection, however, this diagnostic approach may be necessary in patients with clinical characteristics of Giardia infection but negative stool and duodenal fluid specimens. Cytology techniques such as brush cytology or examination of the formalin fixative from tissue samples enhance detection of Giardia over biopsy a**lysis alone.37 The commercially available Entero-Test is an alternative method for obtaining duodenal fluid directly.38

Using polyclonal antisera or monoclonal antibodies against Giardia-specific antigens rather than direct microscopy has improved diagnostic testing for Giardia. Studies comparing EIA kits for detecting Giardia antigen in stool showed a sensitivity of 87% to 100% and a specificity of 100%. All fluorescent antibody tests had 100% sensitivity and specificity.39 These rapid diagnostic tests can be positive before and after detection of organisms by microscopic examination. DFA and EIA were equally sensitive, and both were more sensitive than microscopy of permanently stained smears after concentration in formalin ethyl acetate.40 Specific antibodies to Giardia have been detected and quantified by immunodiffusion, hemagglutination, immunofluorescence, and EIA, but a serologic test is not available commercially.

Commercially available multiplex PCR panels for the detection of GI pathogens, including Giardia, are now available. These tests are highly sensitive (92% to 100%) and specific (96.9% to 100%) and can detect multiple GI pathogens simultaneously.41-43

Prevention Recommendations

Preventing Exposure

Because Giardia organisms are most likely transferred from contaminated water or food, or by contact with an infected person or animal, avoidance of untreated water sources and hand washing with soap and water after exposure to potentially f***lly contaminated material or contact with an infected person or animal are recommended. These recommendations are especially important in individuals with severe immunosuppression. A study in adults with HIV infection in the United States demonstrated the benefits of hand hygiene. In the intervention group, a regimen of intensive hand washing (hand washing after defecation, after cleaning infants who had defecated, before preparing food, before eating, and before and after s*x) coupled with weekly reminder telephone calls regarding hand hygiene resulted in fewer Giardia infections.44 Alcohol-based gels are ineffective against Giardia cysts and should not be used as a substitute for hand washing when exposure to Giardia is a concern.

In a hospital, standard precautions (i.e., use of gloves and hand washing after gloves are removed) should be sufficient to prevent transmission of Giardia from a patient with the infection to a susceptible person with HIV.

Before traveling to areas where the water may be contaminated or the safety of drinking water doubtful, travelers, hikers, and campers should be advised of methods to make water safe for drinking. These measures include using bottled water, disinfecting water by heating it to a rolling boil for 1 minute, or using a filter that has been tested and rated to National Safety Foundation Standard 53 or Standard 58 for cyst and oocyst reduction. Waterborne outbreaks of giardiasis can be prevented with a combination of adequate filtration of water sources, chlorination, and maintenance of water distribution systems.1,9 Travelers should also be advised of the potential for transmission of giardiasis during use of contaminated recreational water (e.g., lakes, rivers, inadequately treated swimming pools).

Preventing First Episode of Disease

No chemoprophylactic regimens are known to be effective in preventing giardiasis. However, because the risk of acquisition of giardiasis and the severity of infection increase with the severity of immunosuppression, ART to prevent or reverse severe immunodeficiency is a primary modality for giardiasis prevention in children with HIV. In the United States, it is standard practice to treat all children with HIV infection with ART.
Treatment Recommendations

Treating Disease

Effective ART and anti-parasitic therapy are the primary initial treatments for Giardia infections in children and adults with HIV infection.21 Supportive care with hydration, correction of electrolyte abnormalities, and nutritional supplementation should be provided. Antimotility agents should be used with caution in young children. Patients with chronic diarrhea should be monitored for malabsorption leading to malnutrition.

The therapeutic efficacy of metronidazole against Giardia led to development of other nitroimidazole derivatives, such as tinidazole and secnidazole. These agents have the advantage of longer half-lives than metronidazole, making them suitable for single-daily-dose therapies. A single, 2-g dose (or the equivalent pediatric dosing of 50 mg/kg in a single dose) of tinidazole has demonstrated cure rates ranging from 80% to 100% and is also associated with improved medication adherence. Cure rates of patients with Giardia have been shown to be consistently higher with the use of tinidazole than with use of other anti-parasitic drugs such as metronidazole, nitazoxanide, mebendazole, albendazole and chloroquine.45-47 Tinidazole is approved for use in children 3 years and older.The drug is available in tablets, which can be crushed in flavored syrup for patients unable to swallow tablets.

Nitazoxanide is approved in the United States for treatment of infections due to G. duodenalis in patients 1 year or older. A randomized, controlled clinical trial in adolescents and adults without HIV infection in Egypt demonstrated nitazoxanide’s efficacy against placebo.48 Nitazoxanide has been compared with metronidazole and mebendazole to treat giardiasis in children and was found to be equally effective, with eradication rates for G. duodenalis of 71% to 81% with nitazoxanide treatment.49

Metronidazole was determined to be therapeutic against giardiasis in 1962. Since then, clinicians have used metronidazole and other nitroimidazoles as the mainstay of therapy of giardiasis. Metronidazole is the drug most often used for giardiasis treatment worldwide. Children have been included in many of the clinical trials of metronidazole, with outcomes similar to those in adults (median efficacy, 94%) with 5- day to 10-day regimens.50 Metronidazole is not available in a standard liquid form, but a suspension can be prepared by thoroughly crushing metronidazole tablets, using glycerin as a lubricant, and suspending the mixture in flavored syrup.51 Despite widespread and accepted use of metronidazole against Giardia, it has not been approved by the U.S. Food and Drug Administration for this indication.

Quinacrine has been used in combination therapy for cases in which treatment failure was suspected.52 The severity of side effects prevented clinicians from using quinacrine as an initial therapeutic choice or first-line alternative, particularly in children. A bitter taste and vomiting led to the drug’s lower efficacy in children, probably because of poor medication adherence.53 Quinacrine is no longer available in the United States and has been discontinued by the manufacturer.54,55

Monitoring and Adverse Events (Including IRIS)

Patients with chronic diarrhea should be closely monitored for signs and symptoms of volume depletion, electrolyte and weight loss, and malnutrition. In severely ill patients, total parenteral nutrition may be indicated.

Adverse effects reported with tinidazole are not as common as with metronidazole but do include bitter taste, vertigo, and GI upset.50

Nitazoxanide is generally well tolerated with no significant adverse events noted in human trials. Adverse events have been mild and transient and principally related to the GI tract, such as abdominal pain, diarrhea, and nausea. Nitazoxanide has been well tolerated up to the maximum dose of 4 g when taken with or without food, but the frequency of GI side effects increases significantly with the dose level.49

The most common side effects of metronidazole treatment include headache, vertigo, nausea, and a metallic taste. Nausea occurs in 5% to 15% of patients given standard multiday courses. In addition, pancreatitis, central nervous system toxicity at high doses, and transient, reversible neutropenia have been attributed to metronidazole.50

Among patients taking quinacrine, 4% to 5% had yellow/orange discoloration of the skin, sclerae, and urine beginning about 1 week after starting treatment, and continuing up to 4 months after the drug was discontinued. Other common side effects of quinacrine included nausea, vomiting, headache, and dizziness. Quinacrine can precipitate hemolysis in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals.53

Immune reconstitution inflammatory syndrome has not been associated with giardiasis or its treatment.

Managing Treatment Failure

The most important steps for management of giardiasis treatment failure are supportive treatment, optimal use of ART to achieve full virologic suppression, and modification of antiparasitic therapy. Treatment failures have been reported with all of the common anti-Giardia agents. It is important that clinicians differentiate between resistance to treatment and reinfection, which is common in Giardia endemic regions and situations where poor hygiene facilitates f***l-oral transmission. Resistance to most anti-Giardia agents has been documented, but there is no consistent correlation between in vitro resistance and clinical failure.50 Clinically resistant strains have been treated with longer repeated courses or higher doses of the original agent or a drug from a different class to avoid potential cross-resistance. Using combination regimens that include metronidazole-albendazole, metronidazole-quinacrine, or other active drugs or giving a nitroimidazole plus quinacrine for at least 2 weeks have both proven successful against refractory infection. Combination therapy with albendazole-praziquantel, nitazoxanide–albendazole, and bacitracin–neomycin has been investigated in clinical trials. However, randomized controlled trials of combination therapy are limited and the optimal combinations need to be clarified, particularly in cases of treatment failure associated with suspected drug tolerance.56 In patients with AIDS who have severe giardiasis, prolonged or combination therapy may be necessary.52,57

Preventing Recurrence

No known pharmacologic interventions effectively prevent recurrence of giardiasis. Reinfection is frequent in endemic areas, or in situations where hygiene is poor or contaminated water (e.g., in private wells) is not adequately treated. Reinfection can be prevented by consistently practicing good hand hygiene, but particularly after defecation and handling of soiled diapers. Hand hygiene should also be practiced before preparing and eating food.12 To reduce risk of disease transmission, children with diarrhea should be excluded from child care settings until the diarrhea has stopped. Children with giardiasis should not frequent recreational water venues for 2 weeks after symptoms resolve. Additional information about recreational water illnesses and how to stop them from spreading is available at
Recommendations
I. In children with HIV infection, what are the best interventions (compared with no intervention) to prevent initial episodes of giardiasis?

Giardiasis can be prevented by practicing good hygiene, not drinking or swimming in water that may be contaminated, and not eating food that may be contaminated (expert opinion).
Because giardiasis results from ingestion of infectious cysts that are passed in the f***s of infected individuals that may contaminate food or water, careful hand washing and washing of fruits and vegetables are recommended.

Frequent hand washing can help reduce the incidence of diarrheal illnesses, including giardiasis (strong, moderate).
A randomized trial of an intensive hand washing intervention (i.e., handwashing after defecation, after cleaning infants who had defecated, before preparing food, before eating, and before and after s*x) in 148 adults with HIV infection in the United States resulted in fewer episodes of diarrheal illness and Giardia infections during a one year period, demonstrating the effectiveness of hand washing.44

Combination antiretroviral therapy of children with HIV infection to reverse or prevent severe immunodeficiency is the primary mode of prevention of severe enteric giardiasis (strong, very low).
A case-control study comparing giardiasis in adults with HIV infection in Brazil before and after the introduction of ART demonstrated that the incidence of enteric diseases caused by Giardia decreased after the introduction of ART.21 Given the evidence, it is reasonable to recommend initiation of ART and immune reconstitution as a primary mode of giardiasis prevention.

II. In children with HIV infection, what are the best interventions (compared with no intervention) to treat giardiasis?

Tinidazole and nitazoxanide are preferred, and metronidazole is the alternative recommended treatment for giardiasis in children (strong, moderate).
Clinical trials in children without HIV infection have demonstrated the efficacy of single dose tinidazole in comparison to other anti-parasitic drugs such as nitazoxanide, mebendazole, albendazole and chloroquine.45-47 Tinidazole can be used in children 3 years and older. Nitazoxanide can be used in children 1 year or older. Metronidazole is inexpensive and widely available and has been used by clinicians as the mainstay of therapy of giardiasis. Metronidazole has been shown to be less efficacious than tinidazole, but comparable to nitazoxanide.7,45,58

Dehydration and electrolyte abnormalities should be corrected (expert opinion).
There are no studies that address this specific management issue in giardiasis. However, recognition and management of hydration status and electrolyte imbalance are key to management of infectious diarrhea.

III. In children with HIV infection, what are the best interventions (compared with no intervention) to prevent recurrent episodes of giardiasis?

Recurrent episodes of giardiasis can be prevented by practicing good hygiene and avoiding contaminated food and water (expert opinion).
Frequent hand washing can help reduce the incidence of diarrheal illnesses, including giardiasis (strong, moderate).
Good hygiene, including frequent hand washing and avoiding contaminated food and water, are recommended to prevent both initial and recurrent Giardia infections.