11/07/2017
Science is amazing!
Snake S*x Determination Dogma Overturned
Researchers find that boas and pythons use an XY s*x chromosome system, rather than the previously assumed ZW system found in some other snakes.
Article by Abby Olena, July 6, 2017
For more than 50 years, scientists have taken for granted that all snakes share a ZW s*x determination system, in which males have two Z chromosomes and females have one Z and one W. But a study, published today (July 6) in Current Biology, reveals that the Central American boa (Boa imperator) and the Burmese python (Python bivittatus) use an XY s*x determination system, which evolved independently in the two species.
“This work is a culmination of a lot of questions that we’ve had about pythons and boas for a long time,” says Jenny Marshall Graves, a geneticist at La Trobe Univeristy in Melbourne, Australia, who did not participate in the study.
Some of these questions came up for Warren Booth, a geneticist and ecologist at the University of Tulsa, as he studied parthenogenesis—the growth and development of offspring in the absence of fertilization. He noticed a pattern for organisms undergoing parthenogenesis: animal species that use a ZW system have only male (ZZ) offspring, and the organisms that use an XY system have only female (XX) offspring. Except this pattern doesn’t hold true for boas and pythons, who consistently produce female offspring by parthenogenesis.
Booth contacted Tony Gamble, a geneticist at Marquette University in Milwaukee, Wisconsin, who studies s*x chromosomes, to begin a collaboration to investigate whether boas and pythons might actually have X and Y chromosomes. Spurred by Booth’s questions, “I went back and reread some of the early papers” on snake s*x chromosomes, says Gamble. “What became clear is that they didn’t show that boas and pythons had a ZW s*x chromosome system. They just said it without any evidence.”
Historically, scientists used light microscopy to photograph and match up homologous chromosomes. “If you find an unmatched pair—two chromosomes that are morphologically different—in males, you have an XY s*x chromosome system. If you find an unmatched pair in females, you have a ZW system,” says Gamble. “But the problem is that a large number of species don’t have s*x chromosomes that are morphologically distinct from each other.”
In order to address this problem, Booth, Gamble, and colleagues digested the genomes of male and female boas, pythons, and Western diamondback rattlesnakes (Crotalus atrox), which are known to use a ZW s*x determination system, with restriction enzymes to create fragments just hundreds of base pairs long. They sequenced the fragments, and then used a computer program to identify s*x-specific genetic markers.
In a ZW system, where females have both a Z and a W, s*x-specific markers will be found in larger numbers in females because they are likely found on the W chromosome, which males don’t have. In an XY system, where males have both an X and a Y, these s*x-specific genetic markers will be found in males because they are likely Y-specific. As expected, the authors identified more markers in sequencing data from rattlesnake females than males. But they also found an excess of s*x-specific genetic markers in male boa and python sequences, which suggested that these snakes have XY s*x determination.
The researchers validated the presence of some of the s*x-specific markers using PCR, and then mapped them to boa and python genome data to confirm which chromosomes were the s*x chromosomes. The team also used the boa and python genomes “to show that, while they both have XY systems, they have actually evolved those XYs independently on different chromosomes,” says Gamble.
The identification of the boa and python s*x chromosomes “might be quite a big breakthrough to our understanding of s*x determination in snakes,” says Graves. “Old fashioned genetic linkage studies will show us where the s*x-linked gene is, and we know enough genomics now to be able to figure out what genes are in that patch of chromosome and ask, are any of them good candidates for s*x determination?”
The authors “found that the python species has different s*x chromosomes than boas, but there are many lineages between them,” says Lukáš Kratochvíl, an evolutionary biologist at Charles University in Prague, Czech Republic, who did not participate in the work. Investigating the s*x chromosomes of these in-between snake lineages could provide insight into the evolution and stability of s*x chromosomes in other animals, he adds.
Gamble agrees that the next step is exploring other species’ s*x chromosome systems, but, for him, a bigger question also arises from this work. “There’s way more going on in snakes than anyone ever thought,” he says. “It was there for anyone to see, and so many scientists—including myself—failed to really look critically at this older literature. One has to wonder how frequently we do this. What other long-held assumptions do I take for granted as factual that could actually not have any empirical evidence behind them?”
LINK: https://goo.gl/jui4RZ
Photo of an axanthic Boa constrictor imperator used with permission.