11/30/2025
A new excerpt from my upcoming novel, A Company of Paws!
The Marvels of Modern Medicine
People sometimes ask outlandish questions when they call looking for information. At the root of their often reasonable but misguided queries is confusion that arises out of misconceptions about veterinary medicine.
“Is this the place where you turn boys into girls?” Cozy always got a huge laugh recounting this caller’s question.
Another favorite: “Do you artificially inseminate dogs? I have a female that I want to breed.”
“Yes, we can do that for you.”
“Do you guys provide the semen?” Cue the laugh track!
Once Cozy managed to compose herself, she answered with a more-or-less straight face. “No, you have to either own the male or find someone who offers their male for stud service.”
“Oh, I don’t have a male dog. I didn’t know you needed one. I just want my female to have one litter of puppies.”
What is the most diplomatic way to respond to that?!
Many dogs do need to be artificially inseminated in order to facilitate breeding. Some breeds, like the Bulldog, have a lot of accumulated genetic deformities that have become acceptable, even desirable traits. But these same sought-after characteristics that define the breed result in mismatched males and females that frequently are unable to copulate naturally. And the female usually requires a caesarian section to deliver the puppies.
Over the years, Dr. Hitchcock gained widespread prominence in our area for treating Bulldogs in general, and especially for breeding. I’ve even found him cited by lay people online, on popular social media sites, as a Bulldog “expert”, which isn’t a real professional designation or certification, but simply an honest reflection of how successful he was at helping owners with the medical and reproductive needs of this breed. We lived in an area directly adjacent to the University, and we were always loyal supporters of the Bulldog Nation.
Bulldogs were in my opinion one of the easiest breeds on which to perform artificial insemination. The males were characteristically enthusiastic about the whole semen collection process. They needed little incentive to participate, and after the first collection, when the clients returned on the following day to repeat the procedure, they often dragged their owners down the hall, beside themselves with excitement and joyfully searching for either Dr. Hitchcock or me. They knew exactly what we were going to do, and they couldn’t wait to see us!
Females, on the other hand, could be a little shy at first. After a semen sample was collected by ej*******ng the male, it was instilled into the reproductive tract of the female. This involved holding her upside down in a “wheelbarrow” position, elevating her hind limbs while her forelegs remained planted firmly on the ground. Once the semen sample was introduced, the female was held in this uncomfortable position for about ten minutes, as gravity facilitated the flow of the precious fluid down into the va**na until it eventually reached the uterus where it would hopefully fertilize her eggs. Females were understandably timid about this process until they had done it a few times.
Usually, artificial insemination involved a guestimate on the owner’s part, of how many days the female had been in “heat”. Normally, we inseminated on days 10, 11, and 12 of the estrous cycle. The average female neared ovulation at about 10 days, but this wasn’t always the case, because no dog is truly average. Typically, we would examine cytology from a va**nal smear when the owners first brought their female in. Using a swab, we scraped the epithelial lining of the va**na and then smeared the collected cells on a slide, where they were stained and observed under the microscope. The cells lining the va**na undergo a change in microscopic appearance as the estrous cycle in the dog progresses toward ovulation. Starting out as plump epithelial cells with large nuclei, the cells begin to undergo a process called cornification, where they change and start to resemble keratinized epithelial skin cells with sharp, angular contours, shrinking amounts of cytoplasm, and a nucleus that decreases in size to a small pinpoint before eventually disappearing altogether. At this point, the cells resemble nothing so much as dried up cornflakes under the microscope, and this guides us in determining the best time to breed. The lay term for va**nal cytology is the “cornflake test”.
Using va**nal cytology is rather subjective and doesn’t always work, and in later years we sometimes tested for rising blood progesterone levels to try to more precisely narrow down the time of ovulation. But we didn’t have the means to measure progesterone levels in-house. A blood sample had to be shipped to an outside lab, and that usually meant the time-sensitive results might not be received for two or three days. If the results indicated ovulation, then the time delay meant it might already be too late to breed by the time we learned the results. So there were drawbacks to both methods, and there were many reasons why a female might not “take” when bred artificially. Frequently it all boiled down to timing of estrus.
Nevertheless, over more than fifty years, Dr. Hitchcock successfully produced hundreds if not thousands of bulldog puppies, as well as pups of other breeds, using only timing and va**nal cytology, and we became well-known for our artificial insemination services. And Bulldog females that became pregnant then needed a caesarian section to deliver the pups. Most females were either too small (Bulldog pups were often large, asymmetrical puppies that might not fit through the birth canal), or the dam would grow weary and not be able to successfully deliver all the pups if the litter were large and the long birthing process were left to progress naturally.
From a young kid of twelve years, I helped Dr. Hitchcock with both artificial insemination and with caesarian sections, and by the time I graduated, I knew exactly how to do it all his way. And who could argue with success?
One morning, Tom Moran came in with one of his female mastiffs. He and his wife, Debbie, bred champion Bullmastiffs that were recognized nationally. The couple had discovered early on that even though the dogs usually bred naturally, without the need for artificial insemination, they still benefited from caesarian sections. The females normally had very large litters, and in their experience, some puppies would be lost simply because the prolonged period required to deliver every pup resulted in some babies dying before they could be born.
Tom and Debbie brought in a beautiful red female named Sadie on this particular visit. Sadie had recently gone into heat, and Tom wanted to inseminate her, but there was a unique twist. The show-winning male he had in mind was actually deceased and had been for twenty years! But the owners of the champion stud had stocked up and saved semen from the dog, and Tom was finally able to procure one of the last surviving frozen samples, at a great cost.
Dr. Hitchcock blew out a deep breath when Tom told him what he planned to do.
“That’s an awful lot of money to spend on such an old sample,” he said. “I don’t think I’ve ever heard of semen frozen for that long being used successfully. Do they provide any warranty or confirmation of its viability?”
Tom shook his head. “They attest to its viability and source, but they don’t offer any kind of guarantee that it will produce pups. Once I purchase it, I won’t be able to get a refund. The only way we can confirm viability is to check it here, right before we inseminate.”
“How many straws do you get?” Dr. Hitchcock asked.
Straws were long, thin plastic pipettes containing a diluted semen sample. If the source were far enough away, and in this case, Tom said the sample was being shipped from Virginia, the straws were packed in ice. For fresh, recently collected samples, the semen itself isn’t frozen, but rather shipped with ice packs or dry ice to keep it chilled until it arrives. If the sample, as in the case of Tom’s prospective sire, were frozen, it was usually overnighted in a nitrogen tank. Either way, the samples were carefully warmed in a liquid water bath just before insemination.
Tom shook his head at Dr. Hitchcock’s question. “I’m paying for one straw. We’ll have just one shot at it.”
“So even the drop we use to check semen viability will decrease the amount of semen available for insemination.”
“Yep, every drop will count.”
“If the quality is good, all you need is a single drop, but still, I know you’re paying an awful lot for the sample,” Dr. Hitchcock replied.
“It’s a gamble, but this dog was from an excellent bloodline, and I’d really like to add that to my gene pool,” Tom said. “I’ve wanted to produce a litter from this male for a long time. It’s been very difficult not to mention expensive to get my hands on a sample.”
We’d successfully inseminated dogs before using shipped semen, and it was almost always chilled. But of course, we’d never used a twenty-year old sample, and from a dead sire no less. Dr. Hitchcock had his doubts we’d be able to produce live pups, but Tom was determined to try.
We agreed to help, and Tom had us perform a cytology smear just to confirm where the dog was in her estrous cycle. Once he confirmed his order, the semen would be shipped overnight. When Dr. Hitchcock examined the stained cytology slide, most of the cells were partially to completely cornified, and it would likely be best to breed the very next day. With this information, the Morans went home and set up delivery for the following morning. The semen would ship directly to the clinic, and when we received it, we’d call Tom to bring Sadie in immediately.
The next morning, the semen sample was delivered right on time. Tom and Debbie lived nearby, a little ways beyond the state border over in Walhalla, South Carolina, and they brought Sadie right over.
We were all excited over the prospect of producing puppies from a sire that had been dead for the last twenty years. If this worked, it would surely constitute some kind of record! At least in our minds it would.
The package contained detailed instructions for how to slowly thaw and warm the sample along with the specialized media it would be mixed with in a water bath of an exact temperature, for a specific length of time. Dr. Hitchcock and I read and reread the instructions before beginning, and we carefully checked the temperature of our warming bath with one of our glass mercury thermometers that we ordinarily used for re**al temperatures. If the sample were allowed to become too hot or cool down below a certain temperature, the s***m cells would be shocked and die.
When the semen sample was ready, Dr. Hitchcock placed the smallest drop from the pipette on a new glass slide and positioned it on the stage of his now ancient microscope. His microscope had originally been purchased new, a lavish gift from his parents when he was accepted into vet school back in 1969. A German model, it was of the finest quality that could be had at the time, and he had taken exceptional care of it over the years. It remained our only clinical microscope, and it had continued to work well with daily use over the last several decades. Only rarely did the lightbulb need replacing.
As Dr. Hitchcock studied the slide, moving it back and forth across the stage, up and down, varying the depth of field, he let out a frustrated sigh. After several minutes, he looked up, shaking his head.
“Tom, I don’t see a single s***m cell in this sample, let alone one that is alive and moving.”
He continued to examine the slide for a few more minutes, hoping to see something. Normally we judged the overall quality of semen samples based on the relative numbers of individual s***m cells, their degree of motility, and the morphology, or shape, of each cell. We wanted to see a slide teeming with uncountable numbers of s***matozoa that moved rapidly across the slide, and we wanted to see few, if any, abnormally shaped heads and tails. But in this case, the shipped sample seemed to be devoid of any s***m cells.
Tom was understandably disappointed at the news. “Well, I’ve paid for the sample, and I don’t have to breed Sadie, so it won’t be the end of the world if she doesn’t take,” he said, rather dejectedly. “Let’s go ahead with it and inseminate the sample. We don’t have anything to lose.”
Sadie was a very large dog, weighing over 125 pounds, and she’d been inseminated before, so she didn’t object when we lifted her backend up off the ground. I sat in a cushioned office chair that was raised as high as possible, then held her rear limbs to either side of my waist, resting most of her weight on my lap. Dr. Hitchcock attached an empty syringe to the flared end of the pipette. Then, after applying copious amounts of lubricating fluid to the v***a and the other end of the pipette, he threaded the opposite end through the lips of the v***a and advanced the tube deep into the va**na. Once the sample was deposited, he withdrew the pipette and held the v***ar lips closed for a few minutes.
I held Sadie upside down in the wheelbarrow position for the next ten minutes, and then Tom and Debbie took her home. They’d return in about seven weeks for a radiograph to see if there were any puppies, but we all had our doubts. The rest of the morning was depressing to say the least. I don’t think either Tom or Debbie had much hope, either.
We didn’t hear from the Morans for several weeks, and I had pretty much forgotten the whole episode. Deep down, I didn’t anticipate we’d have any puppies. It had all seemed a disappointing waste of time and money. But then one morning, rather unexpectedly, Tom and Debbie came breezing through the front door, pulling a very large and very obviously pregnant Sadie on a long leash behind them!
We gathered around in amazement. Sadie’s abdomen was greatly distended, even for a dog her size, and her mammary glands had begun to swell with milk production.
“Has it already been seven weeks, Tom?” Dr. Hitchcock asked in surprise. “Time sure does fly!”
“Yep, seven weeks as of today. I’m positive she’s pregnant, but I wanted to get an idea of how many pups to expect.”
Dr. Hitchcock and I knelt to palpate Sadie’s tremendous abdomen. An enlarged belly doesn’t always mean pregnancy. All female dogs that are not bred during an estrous, or heat cycle, technically go through a false pregnancy. Most females show no evidence of this condition, but some develop a swollen abdomen, produce milk, and will even act out labor and find objects like toys or shoes to “nurse”. But in Sadie’s case, I felt like I could palpate more than one puppy.
We continued to stare at our patient in fascination as Cozy set up the machine for an x-ray and brought a large cassette from the dark room.
“It’s hard to believe she could have that many puppies,” Dr. Hitchcock said. “Especially with such a poor semen sample. But apparently there were a few live s***m cells in there!”
Tom and Debbie heartily agreed, glowing with pleasure at the apparent success of the insemination. All of us couldn’t wait to see the radiograph. The skeletons of canine fetuses start to mineralize and thus show up on x-rays at around 45-49 days of gestation. The total length of pregnancy in dogs is about 63 days on average, so the pups can usually be first detected radiographically at about two weeks prior to birth.
Tom and I helped Dr. Hitchcock lift the giant dog up onto the treatment table and then coax her to lie on her side for a lateral view of her abdomen. Dr. Hitchcock adjusted the collimator light on the x-ray unit, centering the beam on the distended belly rising before us like a massive mountain. After measuring the height of Sadie’s abdomen, he calculated the exposure settings and adjusted the dials on the mobile x-ray unit we used.
About fifteen minutes later, I removed the developed film from the darkroom, still dripping water from the last rinse, and brought it out to the view box where everyone waited impatiently.
There, plain to see, were a great many number of rather large puppies, their fish-like backbones all tangled together in the enormous abdominal cavity, resembling nothing so much as a platter of fish served up on the comic strip Garfield. We usually counted the rounded skulls in order to estimate the number of pups, but an exact count wasn’t really needed as we planned a caesarian section anyway.
“Phew, Tom! That is a large litter. How many pups do you count, Jason?” Dr. Hitchcock asked.
“I see at least eleven heads,” I replied.
“That’s how many I see, too!” he agreed.
Tom and Debbie grinned with pride, and Sadie, glad to finally be back on the solid tiled floor, wagged her head and joined in the general merriment. It was hard to believe the dog was pregnant, let alone with eleven pups! And from a twenty-year-old semen sample that didn’t seem to have had any viable s***m at all!
We scheduled Sadie’s surgery for two weeks later, and when the day arrived, the Morans pulled up to the clinic early that morning, pregnant dog in tow. Her protruding abdomen, impossibly larger by now, sagged close to the ground. Tom led Sadie in, the reluctant animal moving decidedly slower today, while Debbie brought up the rear with an oversized basket filled with towels and a heating pad. The couple had helped with many caesarian sections over the years and were old hands at the procedure.
Dr. Hitchcock administered a preanesthetic, although for caesarian sections, we always omitted the use of a tranquilizer. With general anesthesia, the newborns would already be somewhat depressed when they were delivered, and we avoided any sedation to try to minimize the additional depressive effects on the babies.
From years of experience, we had perfected our process for the surgery. We employed a long-tested, tried and true team effort, and in no time, Sadie was up on the table, anesthetized, and positioned on her back. Dr. Hitchcock quickly shaved the hair and prepped the abdomen with surgical scrub, coating it with a final layer of the dark brown povidone iodine antiseptic solution. The surgery pack was opened and positioned on the instrument tray at the foot of the surgery table, with sterile packs of suture and scalpel dumped out onto the drape of the pack. He and I gloved up and draped off the abdomen, and literally within minutes of inducing anesthesia, the dog’s abdomen was opened, the large, bicornuate uterus carefully lifted from the abdomen, one horn at a time, and splayed open on the sterile drape like a lumpy oversized letter “Y”. The massive organ required several additional hands under the drape to support each arm of the uterus while we extracted the pups one by one. Time was of the essence; the longer it took to get the puppies out, the more depressed they could be, and the longer it would take to revive them.
Making an incision near the confluence of the two horns of the uterus, we removed the first pup and handed it off to Cozy. Standing at the nearby sink, she deftly stripped away the placental membranes, clamped and severed the umbilical cord, then handed the damp newborn off to one of our assistants. By the time she had finished with the first pup, we had pushed the next pup along one horn of the uterus and out of the incision. One by one, each pup was delivered and the uterus, deprived of its precious cargo, slowly shrunk dramatically in size. The timing for delivery had been precise, since we knew the exact and only date of insemination. Each pup was already wriggling before it could be liberated from the fluid-filled amniotic sacs surrounding it, and in no time the raucous sound of crying puppies filled the air. These puppies needed little stimulation to begin to breathe, and as they filled their lungs with new air, their faces and feet rapidly turned a brilliant pink color from oxygenation.
Once each of the eleven pups were delivered, the crew assisting in the room next door wiped it down, aspirating the mouth and nostrils with a bulb syringe and slinging the puppy upside down through the air in a wide arc while supported to clear any remaining fluid from the airway. Finally, after drying the baby off, the umbilical cord was tied with a length of suture and a healthy dose of antiseptic applied to the raw end. Each puppy then joined its siblings in Debbie’s large cozy basket. The growing mound of squealing newborns thrashed about under the heated towels, with additional warmth provided by a heating pad set on low underneath the basket.
Meanwhile, back in the surgery room, I assisted Dr. Hitchcock with closing the incision in the uterus. Using absorbable suture, he drew the edges of the defect together with a simple continuous pattern. Once he reached the end of the incision, he doubled back and inverted the original incision to reduce the possibility of adhesions. We replaced the uterus in the abdominal cavity, and Dr. Hitchcock quickly closed the abdomen by suturing together the linea alba, followed by the subcutaneous tissue. Using a Ford interlocking pattern, he neatly apposed the skin, and then we turned Sadie onto her side. We were done in record time, and the healthy screams of the vigorous litter of puppies next door punctuated the end of the surgery. I turned the gas anesthesia vaporizer off, and within minutes, Sadie was already swallowing and chewing at her endotracheal tube. We extubated her, and with Tom’s help lifted her onto a blanket on the floor. Soon, our recently anesthetized patient was already trying to stand to her feet. Wobbling, she began to make her way toward the chorus of cries from her newly born litter of puppies.
Tom and Debbie were more than happy with the results. Despite the low chance of success, Tom’s gamble had improved an already excellent mastiff bloodline, all with a progenitor that no longer lived. I’d never seen pups born to a dead sire, let alone one that had died twenty years before! As I thought about it, the father of this litter of pups died when I was still a young boy! Although a fairly common procedure now, artificial insemination and the use of frozen semen in dogs had still been a relatively newer development, rarely done, back when our sire was collected two decades before. In fact, Tom told us that the semen samples were obtained and stored at the veterinary school at Virginia Tech. And yet, somehow that semen had remained viable today, all these years later. What a miracle of modern medicine indeed! It was all very impressive, even if I’d helped just a little bit!
We never forgot the unbelievable story of the twenty-years deceased s***m donor that fathered a beautiful litter of thriving Bullmastiffs, and we’ve told this tale many times over the years. There have been other instances, too, where we inseminated dogs with frozen or chilled semen samples that seemed to be of poor quality, and yet the females went on to have rather large litters. The lesson learned here was to never underestimate the power of a single s***m cell. A normal sample may have hundreds of millions of individual s***m cells, so even a poor sample can’t be discounted! Anything is possible and against all odds, life usually finds a way!
Excerpt from A Company of Paws © 2025 Jason K. Macomson All Rights Reserved
A Company of Paws is expected to be released next summer. In the meantime, if you haven't read it yet, look for my first novel about veterinary medicine, Red Barn Tales, available on Amazon or many other online retailers!
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